038 Autoantibody landscape of cutaneous disorders revealed by wet protein array

Autoantibodies are found in various pathological conditions such as autoimmune diseases, infectious and malignant tumors. However, their clinical implications have not yet been fully elucidated. Herein, we employed our proteome-wide cDNA library (HuPEX) which covers approximately 90% of the human transcriptome for autoantibody evaluation. In first step, screening was conducted upon pooled serum. second concentration selected autoantibodies within individual sera quantified. Primary identified a total 565 three representative inflammatory disorders: systemic sclerosis (SSc), psoriasis, cutaneous arteritis. Quantitative analysis revealed that each level either positively or negatively correlated with serum levels C-reactive protein. SSc, discovered 18 whose Enrichment significantly high proportion proteins targeted by these regulated SNAI1, transcription factor known to be upregulated SSc skin. Sum associated manifestations SSc. Similar results were obtained psoriasis addition, 488 detected from melanoma. Notably, patients metastases had increased overall production compared those tumors limiting primary site. Collectively, novel technology can reveal “autoantibody landscape” subjects may provide biomarkers.